SAHA和TRAIL联合应用对雌激素受体阳性乳腺癌细胞MCF-7生长抑制的分子机制

冯秀艳,晏林,周伟强

中国药学杂志 ›› 2016, Vol. 51 ›› Issue (16) : 1373-1378.

PDF(2468 KB)
PDF(2468 KB)
中国药学杂志 ›› 2016, Vol. 51 ›› Issue (16) : 1373-1378. DOI: 10.11669/cpj.2016.16.007
论 著

SAHA和TRAIL联合应用对雌激素受体阳性乳腺癌细胞MCF-7生长抑制的分子机制

  • 冯秀艳1,2,晏林3,周伟强1*
作者信息 +

Effects of Combination with SAHA and TRAIL Treatment on Cell Growth of ER Positive Breast Cancer Cell Line MCF-7

  • FENG Xiu-yan 1,2,YAN Lin 3,ZHOU Wei-qiang 1*
Author information +
文章历史 +

摘要

目的 探讨SAHA(suberoylanilide hydroxamic acid)和肿瘤坏死因子相关凋亡诱导配体(TNF-related apoptosis inducing ligand,TRAIL)联合作用抑制雌激素受体阳性乳腺癌细胞MCF-7生长的分子机制。方法 以乳腺癌细胞株MCF-7为研究对象,应用自动细胞分析仪测定SAHA和TRAIL联合作用对乳腺癌细胞活力和细胞凋亡的影响并对细胞周期进行分析;应用实时定量PCR及固相凋亡抗体芯片检测MCF-7细胞凋亡蛋白表达。结果 SAHA和TRAIL联合作用可显著降低MCF-7细胞活力,抑制细胞增殖,并诱导细胞凋亡的产生。SAHA和TRAIL对MCF-7细胞周期的影响主要集中在G0/G1期。结论 SAHA和TRAIL联合作用对乳腺癌细胞生长有抑制作用。

Abstract

OBJECTIVE To study the effects of suberoylanilide hydroxamic acid (SAHA) and TRAIL treatment on cell proliferation and apoptosis for ER positive breast cancer cell line MCF-7. METHODS Human breast cell lines (MCF-7) were evaluated for the expressions of cell viability,cell apoptosis and cell cycle by muse cell analyzer. The mRNA levels of related apoptotic factors in MCF-7 cells were detected by real time PCR and solid phase apoptosis antibody microarray. RESULTS After the combination with SAHA and TRAIL,the ability of cell proliferation and cell viability were depressed,and the cell apoptosis was induced. The cell cycle assay showed that the MCF-7 cells were arrested in G0/G1 phase with SAHA and TRAIL treatment. CONCLUSION The combinatorial treatment of SAHA and TRAIL has a significantly inhibitory effect on cell growths of ER positive breast cancer MCF-7 cell.

关键词

雌激素受体阳性乳腺癌 / 细胞凋亡 / SAHA / 肿瘤坏死因子相关凋亡诱导配体

Key words

estrogen receptor positive breast cancer / cell apoptosis / suberoylanilide hydroxamic acid (SAHA) / TNF-related apoptosis inducing ligand(TRAIL)

引用本文

导出引用
冯秀艳,晏林,周伟强. SAHA和TRAIL联合应用对雌激素受体阳性乳腺癌细胞MCF-7生长抑制的分子机制[J]. 中国药学杂志, 2016, 51(16): 1373-1378 https://doi.org/10.11669/cpj.2016.16.007
FENG Xiu-yan,YAN Lin,ZHOU Wei-qiang. Effects of Combination with SAHA and TRAIL Treatment on Cell Growth of ER Positive Breast Cancer Cell Line MCF-7[J]. Chinese Pharmaceutical Journal, 2016, 51(16): 1373-1378 https://doi.org/10.11669/cpj.2016.16.007
中图分类号: R965   

参考文献

[1] MOORE E K,ROYLANCE R,ROSENTHAL A N. Breast cancer metastasising to the pelvis and abdomen: what the gynaecologist needs to know [J]. BJOG, 2012,119(7):788-794.
[2] ZHOU W,WANG G,GUO S. Regulation of angiogenesis via Notch signaling in breast cancer and cancer stem cells [J]. Biochim Biophys Acta,2013,1836(2):304-320.
[3] ERIN K S,WEI X. Selectively targeting estrogen receptors for cancer treatment [J]. Adv Drug Deliv Rev, 2010,62(13): 1265-1276.
[4] HEFTI M M,HU R,KNOBLAUCH N W, et al. Estrogen receptor negative/progesterone receptor positive breast cancer is not a reproducible subtype [J]. Breast Cancer Res, 2013,15(4):P68.
[5] LEO K,ANNA S,PAMELA M D,et al. Combining histone deacetylase inhibitor vorinostat with aurora kinase inhibitors enhances lymphoma cell killing with repression of c-myc,hTERT and microRNA levels [J]. Cancer Res,2011,71(11): 3912-3920.
[6] WILEY S R,SCHOOLEY K,SMOLAK P J, et al. Identification and characterization of a new member of the TNF family that induces apoptosis [J]. Immunity, 1995,3(6):673-682.
[7] FADEEV R S,CHEKANOV A V,DOLQIKH N V,et al. Inhibitor sorafenib and HDAC inhibitor suberoylanilide hydroxamic acid suppress confluent resistance of cancer cells to recombinant protein izTRAIL [J]. Biofizika,2012,57(4):655-661.
[8] LAURICELLA M,CIRAOLO A,CARLISI D,et al. SAHA/TRAIL combination induces detachment and anoikis of MDA-MB231 and MCF-7 breast cancer cells [J]. Biochimie,2012,94(2):287-299.
[9] LEE C K,WANG S,HUANG X, et al. HDAC inhibition synergistically enhances alkylator-induced DNA damage responses and apoptosis in multiple myeloma cells [J]. Cancer Lett,2010,296(2):233-240.
[10] CUI D D,HUANG Y,MAO S H, et al. Synergistic antitumor effect of TRAIL and adriamycin on the human breast cancer cell line MCF-7 [J]. Braz J Med Biol Res,2009,42(9):854-862.
[11] LEE Y J,WON A J,LEE J,et al. Molecular mechanism of SAHA on regulation of autophagic cell death in tamoxifen-resistant MCF-7 breast cancer cells [J]. Med Sci, 2012,9(10):881-893.
[12] GAO W,MEI X,WANG J,et al. ShRNA-mediated knock-down of CXCR7 increases TRAIL-sensitivity in MCF-7 breast cancer cells [J]. Tumour Biol,2015,36(9):7243-7250.
[13] CONNOLLY D,YANG Z,CASTALDI M,et al. Septin 9 isoform expression,localization and epigenetic changes during human and mouse breast cancer progression [J]. Breast Cancer Res,2011,13(4):R76.
[14] THUSHANGI N P,VERED S,STEFFI O. Epigenetic regulation in estrogen receptor positive breast cancer-role in treatment response [J] J Mammary Gland Biol Neoplasia,2010,15(1): 35-47.

基金

国家自然科学基金资助项目(81172509);沈阳医学院科技基金项目(20121003)

PDF(2468 KB)

Accesses

Citation

Detail

段落导航
相关文章

/